BiologyAQA, Edexcel and OCR
Looking at cells with a microscope
Make a slide of plant or animal cells, look at it under a light microscope, then draw and label what you see.
- AQARequired practical 1 (Biology), 1 (Combined)
- EdexcelCore practical, Biology and Combined Science
- OCRPAG: microscopy, Biology and Combined Science
- Method in short
- Peel a thin layer of onion skin and lay it flat in a drop of water on a slide.
- Add a drop of iodine solution to stain it, then lower a cover slip at an angle so no air bubbles get trapped.
- Start with the lowest power objective lens and focus with the coarse focusing knob.
- Switch to a higher power and sharpen the image with the fine focusing knob.
- Draw a few cells with a sharp pencil, label the cell wall, cytoplasm and nucleus, and note the magnification.
- What you should find
- Total magnification is the eyepiece lens magnification multiplied by the objective lens magnification. To find the real size of a cell, divide the size of the image by the magnification.
- Stay safe
- Iodine solution irritates eyes and stains skin, so wear eye protection. Handle slides and cover slips with care, as they break into sharp pieces.
- Where marks go
- Drawings should use clear single lines with no shading, and label lines drawn with a ruler that don’t cross.
BiologyAQA, Edexcel and OCR
Antiseptics and antibiotics on bacteria
Find out which antiseptic or antibiotic is best at stopping bacteria from growing.
- AQARequired practical 2 (Biology only)
- EdexcelCore practical, Biology only
- OCRPAG: microbiological techniques, Biology and Combined Science
- You change
- The antiseptic or antibiotic on each paper disc.
- You measure
- The width of the clear zone around each disc, where bacteria haven’t grown.
- Keep the same
- The size of the discs, how long they soak, the bacteria, the agar, and the temperature and time of incubation.
- Method in short
- Pass an inoculating loop through a Bunsen flame to sterilise it, and let it cool.
- Spread bacteria evenly over the agar in a Petri dish.
- Put discs soaked in each substance on the agar, plus one soaked in sterile water as a control.
- Tape the lid on with a few strips, label the dish and incubate it upside down at no more than 25 °C.
- After about two days, measure the clear zone around each disc and work out its area.
- What you should find
- The bigger the clear zone, the better that substance is at killing the bacteria or stopping them growing.
- Stay safe
- Keep everything sterile and never open the dish after incubating it. Incubating at 25 °C or below makes it less likely that bacteria harmful to people will grow.
- Where marks go
- Don’t seal the lid all the way round: oxygen needs to get in, so that harmful bacteria that grow without oxygen don’t take over.
BiologyAQA, Edexcel and OCR
Osmosis in plant tissue
Find out how the concentration of a sugar or salt solution affects the mass of pieces of potato.
- AQARequired practical 3 (Biology), 2 (Combined)
- EdexcelCore practical, Biology and Combined Science
- OCRPAG: transport in and out of cells, Biology only
- You change
- The concentration of the solution, including one tube of pure water.
- You measure
- The mass of each potato cylinder before and after, turned into a percentage change in mass.
- Keep the same
- The size of the cylinders, the volume of solution, the time they are left and the temperature.
- Method in short
- Cut cylinders of potato with a cork borer and trim them to the same length.
- Blot each one dry, weigh it on a balance and write the mass down.
- Put one cylinder in each tube of solution and leave them all for the same time.
- Take them out, blot them dry in the same way and weigh them again.
- Work out the percentage change in mass and plot it against the concentration.
- What you should find
- In weak solutions the potato gains mass, because water moves into the cells by osmosis. In strong solutions it loses mass. Where the line crosses zero, the solution matches the concentration inside the cells.
- Stay safe
- Cut onto a tile, with the blade moving away from your fingers.
- Where marks go
- Use percentage change, not just the change in mass, because the cylinders don’t all start at exactly the same mass.
BiologyAQA, Edexcel and OCR
Food tests
Find out which food molecules (starch, sugars, proteins and fats) are in a food.
- AQARequired practical 4 (Biology), 3 (Combined)
- EdexcelCore practical, Biology only
- OCRPAG: testing for biological molecules, Biology only
- Method in short
- Grind the food with a little distilled water, then filter it to get a solution.
- Starch: add iodine solution. It turns from orange-brown to blue-black if starch is there.
- Sugars: add Benedict’s solution and heat in a hot water bath for a few minutes. Blue turns green, yellow or brick-red if a reducing sugar such as glucose is there.
- Protein: add Biuret solution. Blue turns purple if protein is there.
- Fats (the emulsion test, which Edexcel names): shake the food with ethanol, then pour the liquid into water. A cloudy white layer means fat is there. Some schools use Sudan III instead, which stains fat red.
- What you should find
- Each test is either positive or negative. With Benedict’s, the colour also hints at how much sugar there is, from green for a little to brick-red for a lot.
- Stay safe
- Wear eye protection. Heat Benedict’s in a water bath, never over a flame, and keep ethanol well away from flames.
- Where marks go
- Always give the colour before and after, and remember that Benedict’s needs heating while the others don’t.
BiologyAQA, Edexcel and OCR
Enzymes and pH
Find out how pH affects how quickly the enzyme amylase breaks down starch.
- AQARequired practical 5 (Biology), 4 (Combined)
- EdexcelCore practical, Biology and Combined Science
- OCRPAG: rates of enzyme-controlled reactions (pH or another factor, such as temperature), Biology and Combined Science
- You change
- The pH, using a different buffer solution each time.
- You measure
- The time until all the starch has gone.
- Keep the same
- The temperature (in a water bath) and the volumes and concentrations of amylase and starch.
- Method in short
- Put a drop of iodine solution in each dip of a spotting tile.
- Warm the amylase, the starch and a buffer solution in a water bath.
- Mix them and start the stopwatch.
- Every 30 seconds (AQA’s interval), put a drop of the mixture onto a fresh drop of iodine.
- When the iodine stays orange-brown, the starch has gone: write down the time. Repeat at other pH values.
- What you should find
- The starch goes fastest at the enzyme’s best (optimum) pH. Far from it, the active site changes shape, the enzyme is denatured and starch no longer fits.
- Stay safe
- Wear eye protection, as iodine solution and enzyme solutions can irritate.
- Where marks go
- You only know the time to the nearest sampling interval, so testing more often gives a more precise result.
BiologyAQA, Edexcel and OCR
Light intensity and photosynthesis
Find out how light intensity affects the rate of photosynthesis in pondweed or algal balls.
- AQARequired practical 6 (Biology), 5 (Combined)
- EdexcelCore practical, Biology and Combined Science (algal balls in hydrogencarbonate indicator, which changes colour as carbon dioxide is taken in)
- OCRPAG: photosynthesis, Biology and Combined Science
- You change
- The distance of the lamp from the pondweed.
- You measure
- The number of oxygen bubbles given off in a minute, or the volume of gas collected.
- Keep the same
- The temperature (an LED lamp gives off little heat), the amount of carbon dioxide (the same sodium hydrogencarbonate solution), and the same piece of pondweed.
- Method in short
- Put pondweed in a boiling tube of water with a little sodium hydrogencarbonate.
- Measure the distance from the lamp to the pondweed with a ruler.
- Leave the pondweed a few minutes to settle at that light level.
- Count the bubbles given off in one minute, and repeat to find a mean.
- Move the lamp to a new distance and repeat.
- What you should find
- The closer the lamp, the faster the bubbles, until something else such as carbon dioxide or temperature limits the rate. On Higher, you also need to know that light intensity is proportional to 1 ÷ distance² (the inverse square law).
- Stay safe
- Lamps get hot. Keep water away from the lamp and the plug, and mop up spills straight away.
- Where marks go
- Bubbles vary in size, so collecting the gas in a syringe or a measuring cylinder is more accurate than counting.
BiologyAQA
Reaction time
Find out how a factor, such as practice or a caffeine drink, affects how quickly a person reacts.
- AQARequired practical 7 (Biology), 6 (Combined)
- You change
- The factor you are testing.
- You measure
- How far a dropped ruler falls before it is caught, turned into a reaction time with a table.
- Keep the same
- The person, the hand they use and where the ruler starts.
- Method in short
- Your partner holds a ruler upright, with the zero mark level with the top of your thumb.
- They let go without warning, and you catch it as fast as you can.
- Read the distance at the top of your thumb.
- Repeat several times and work out a mean.
- Change the factor, then repeat the whole test.
- What you should find
- A shorter distance means a faster reaction time. Practice usually makes people faster.
- Stay safe
- Low risk. If you test a caffeine drink, check that no one taking part needs to avoid caffeine.
- Where marks go
- People get tired or improve with practice, so things are hard to keep the same: repeat and take a mean.
BiologyEdexcel and OCR
Rate of respiration
Find out how temperature affects the rate of respiration in small living things, such as woodlice or germinating peas.
- EdexcelCore practical, Biology and Combined Science
- OCRPAG: physiology, responses and respiration, Biology only (OCR’s example is exercise and pulse rate, so a respirometer is just one option)
- You change
- The temperature of the water bath.
- You measure
- How far the coloured liquid moves along the capillary tube in a set time.
- Keep the same
- The number or mass of organisms, the amount of soda lime and the time.
- Method in short
- Put soda lime in a boiling tube to absorb carbon dioxide, then a gauze on top.
- Put the organisms on the gauze and fit a capillary tube with a drop of coloured liquid.
- Stand the tube (a respirometer) in a water bath at the first temperature.
- Measure how far the liquid moves in a set time, such as five minutes.
- Repeat at other temperatures.
- What you should find
- The organisms take in oxygen and the carbon dioxide they give out is absorbed, so the air in the tube shrinks and the liquid moves towards them. The faster it moves, the faster they are respiring.
- Stay safe
- Soda lime is corrosive, so wear eye protection and don’t touch it. Handle animals gently and return them to where you found them.
- Where marks go
- Set up a control tube with glass beads instead of organisms, to show that the movement is caused by the living things.
BiologyAQA and OCR
Light or gravity and seedling growth
Find out how light from one side, or gravity, affects the way young seedlings grow.
- AQARequired practical 8 (Biology only)
- OCRTwenty First Century Biology only: not a PAG, but you may be asked to describe light experiments on shoots
- You change
- The direction of the light (or which way up the seedlings are).
- You measure
- The direction the shoots or roots grow in, and how long they get.
- Keep the same
- The type and number of seeds, the water, the temperature and the time.
- Method in short
- Grow cress or bean seeds on damp cotton wool in Petri dishes.
- Put one dish in a box lit from one side only, and one in even light as a control.
- For gravity, turn some seedlings on their side, kept in the dark.
- After a few days, measure the shoots and roots and make labelled drawings to show which way they are growing.
- What you should find
- Shoots grow towards light (phototropism), because the hormone auxin builds up on the shaded side and makes cells there grow longer. Roots grow downwards with gravity (gravitropism).
- Stay safe
- Low risk. Wash your hands after handling seeds and cotton wool.
- Where marks go
- Use the right name for each response, phototropism or gravitropism, and say what auxin does.
BiologyAQA, Edexcel and OCR
Sampling with quadrats and transects
Estimate the size of a population with random quadrats, and see how a species’ spread changes along a transect.
- AQARequired practical 9 (Biology), 7 (Combined)
- EdexcelCore practical, Biology and Combined Science
- OCRPAG: sampling techniques, Biology and Combined Science
- You change
- Where the quadrat goes along the transect (and so a factor such as light or trampling).
- You measure
- The number of plants of one species, or the percentage of the quadrat they cover.
- Keep the same
- The size of the quadrat and the way you count.
- Method in short
- Lay two tape measures at right angles to make a grid over the area.
- Use random numbers to pick coordinates, place a quadrat there and count the species.
- Do this for many quadrats and find the mean number per quadrat.
- Scale up: mean per square metre × area in square metres gives the estimated population.
- For a transect, lay a tape across the area and place a quadrat at regular points along it.
- What you should find
- Random quadrats give a fair estimate of a whole population. A transect shows how numbers change from one place to another, for example from shade into sun.
- Stay safe
- Check the site for hazards such as water, steep ground or stinging plants, and wash your hands afterwards.
- Where marks go
- Random placing avoids picking spots by eye, and more quadrats give a more reliable estimate.
BiologyAQA
Temperature and the decay of milk
Find out how temperature affects how fast milk goes off, by following the change in pH.
- AQARequired practical 10 (Biology only)
- You change
- The temperature of the water bath.
- You measure
- The time for the pH to fall, shown by an indicator changing colour.
- Keep the same
- The volumes of milk, enzyme (lipase) and sodium carbonate solution, and the amount of indicator.
- Method in short
- Put milk, sodium carbonate solution and a few drops of an indicator that is pink in alkali into a test tube.
- Warm it and a tube of lipase in a water bath to the chosen temperature.
- Add the lipase and start the stopwatch.
- Stop the clock when the pink colour disappears.
- Repeat at other temperatures.
- What you should find
- Lipase breaks fats into fatty acids, which lower the pH. It works faster as the temperature rises, up to its best temperature, then slows as the enzyme is denatured.
- Stay safe
- Never drink the milk. Wear eye protection, as sodium carbonate solution irritates the eyes.
- Where marks go
- Judging the end of a colour change is tricky, so compare against a tube of the final colour.